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SRX681906: GSM1480233: Stationary Nat +CMC; Saccharomyces cerevisiae; OTHER
1 ILLUMINA (Illumina Genome Analyzer II) run: 7.6M spots, 275M bases, 144.1Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: PSI-seq to Identify Sites of Pseudouridylation in S. cerevisiae
PRJNA258278 • SRP045530 • All experiments • All runs
show Abstracthide Abstract
Using a technique based on the ability of CMC to specifically label pseudouridines and to stop reverse transcriptase, we provide a transcriptome-wide map of pseudouridylation in S. cerevisiae under log phase and heat shock conditions Overall design: 4 conditions: log phase (2x), heat shock, and stationary phase. For each condition, there are 4 libraries: natural RNA + or - CMC treatment and in vitro transcribed RNA + or - CMC treatment
Sample: Stationary Nat +CMC
SAMN02991363 • SRS685462 • All experiments • All runs
Organism: Saccharomyces cerevisiae
Library:
Instrument: Illumina Genome Analyzer II
Strategy: OTHER
Source: TRANSCRIPTOMIC
Selection: other
Layout: SINGLE
Construction protocol: Hot phenol chloroform extraction, 2x poly(A) purification with Ambion polyA Purist Kit PSI-seq. Libraries were constructed as described in Lovejoy et al.
Experiment attributes:
GEO Accession: GSM1480233
Links:
External link:
Runs: 1 run, 7.6M spots, 275M bases, 144.1Mb
Run# of Spots# of BasesSizePublished
SRR15523697,640,036275M144.1Mb2015-07-22

ID:
954236

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